Patent · US Expired

Immunoassay microparticle washing system and method of use

US4486315A · kind A · utility

56Cited by
17References
10Claims
0Family size

Assignee

Inventor

Key dates

Filing dateMar 11, 1982
Grant dateDec 4, 1984
Priority date
Expiry dateMar 11, 2002

Classification

  • Technology area (CPC Y)Emerging Cross-Sectional Technologies
  • CPC primaryY10T436/25375
  • WIPO fieldChemical engineering
  • WIPO sectorChemistry

Abstract

A particle washing system and method of use provides for the placement of the fluid containing the desired particles within an inner tube having near the bottom thereof an orifice plugged with a material for sealing the particulate containing fluid from a wash fluid under forces substantially equal to 1G. The inner tube is positioned within an outer tube which contains a wash fluid having a density at least equal to that of the particle containing solution but less than that of the particles. Application of centrifugal force, substantially greater than 1G, and directed toward the bottom of the outer tube, causes the sealing material to be dislodged thereby permitting the particles to move through the orifice and through the outer solution. Thus the particles are collected from the inner solution, washed by the outer solution, and pelleted at the bottom of the outer tube.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.