Enzyme/immunofluorescent assay for autoantibodies
US4487830A · kind A · utility
25Cited by
1References
9Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | May 14, 1982 |
| Grant date | Dec 11, 1984 |
| Priority date | — |
| Expiry date | May 14, 2002 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2800/24
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for the determination of autoantibody in a test sample comprises contacting a substrate for the autoantibody with sample; treating the contacted substrate with labeled antihuman antibody selected from (1) a mixture comprising enzyme labeled antihuman antibody and fluorescent labeled antihuman antibody, and (2) antihuman antibody labeled with an enzyme and a fluorescent label; determining the enzyme activity of the treated substrate; and determining the immunofluorescent patterns in substrates exhibiting enzyme activity. The method is useful for the rapid screening and characterization of autoantibodies.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.