Immunoassay methods employing patterns for the detection of soluble and cell surface antigens
US4487839A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Jan 5, 1983 |
| Grant date | Dec 11, 1984 |
| Priority date | — |
| Expiry date | Jan 5, 2003 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/823
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Methods for determining the presence of antigens or antibodies in an aqueous sample or presence of antigens on the surface of cells. A preferred embodiment employs fluorescent antigens which compete with the sample antigens for antibody binding sites. The antibodies are deposited on a support surface means in alternating patterns. The surface means and fluorescence detector are translocated with respect to each other and a signal generated by the detection of the repeating pattern of fluorescence. The signal is analyzed by means of a gated integrator responsive to a gate track control means also located on the surface means. Immunoassay methods having increased sensitivity are thereby obtained.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.