Method for discriminating between unstained and absorbing dye stained cells
US4492752A · kind A · utility
60Cited by
10References
3Claims
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Key dates
| Filing date | Sep 3, 1982 |
| Grant date | Jan 8, 1985 |
| Priority date | — |
| Expiry date | Sep 3, 2002 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/805
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Method for discriminating unstained cells from stained cells in a heterogeneous population. Specified cell types are stained with an absorbing stain and all cells are passed through the class of flow cytometry instrumentation employing focused collimated light sources. Detection of low angle and wide angle light scatter permits differentiation between the cell types on the basis that cells stained with an absorbing stain produce comparatively less low angle light scatter and comparatively more wide angle light scatter than do unstained cells.
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