Composition and method for the determination of hydrogen peroxide

US4492754A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Ulrich Trager · Schifferstadt, DE
• Bernward Sojka · Viernheim, DE
• Hans Lange

Key dates

• Filing date: Jun 11, 1982
• Grant date: Jan 8, 1985
• Expiry date: Jun 11, 2002

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10T436/206664
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Composition for the detection of PA1 (a) hydrogen peroxide or hydrogen peroxide forming substrates or PA1 (b) peroxidases or peroxidatively reacting substances which composition comprises a substrate capable of coupling and an oxidizable substance known to undergo the Trinder reaction, and PA1 (a) a peroxidase or PA1 (b) hydrogen peroxide wherein the substrate capable of coupling is a compound of the formula ##STR1## in which n and m are individually selected from whole numbers of 1 to 4, PA1 X and Y, which can be the same or different represent a valence bond or a phenylene radical, PA1 R.sub.1 and R.sub.2 are individually selected from carboxyl or sulfonic acid groups and one of R.sub.1 and R.sub.2 can also be hydrogen or lower alkyl, and PA1 R.sub.3 and R.sub.4 are individually selected from hydrogen and alkyl radicals of up to 6 carbon atoms; and/or a salt of such compound. The present invention also provides a method wherein the composition is brought together with a sample and the coloration obtained is evaluated visually or with a photometer.