Cloned high signal strength promoters

US4495280A · kind A · utility

Assignee

• The Board of Trustees of the Leland Stanford Jr. University · US

Inventors

• Hermann Bujard · Heidelberg, DE
• Annie C. Y. Chang · Palo Alto, US
• Stanley N. Cohen · Stanford, US

Key dates

• Filing date: May 20, 1981
• Grant date: Jan 22, 1985
• Expiry date: May 20, 2001

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/70
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Method for preparing high signal strength promoters and terminators and DNA compositions employing such promoters and terminators. T5 phage is cleaved to provide for DNA sequences having intact promoters. These promoters are inserted into vectors separated from a balanced terminator by a gene of interest and the terminator is desirably followed by a marker allowing for selection of transformants. High efficiencies in transcription of DNA can be achieved with the highly active T5 promoters. The promoters and terminators are used in hybrid DNA for efficient expression of structural genes and transcription to provide RNA sequences.