Liquid proteinase concentrate and method for preparation
US4497897A · kind A · utility
7Cited by
4References
7Claims
0Family size
Assignee
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Key dates
| Filing date | Dec 9, 1982 |
| Grant date | Feb 5, 1985 |
| Priority date | — |
| Expiry date | Dec 9, 2002 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/96
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A storage stable liquid enzyme concentrate of Subtilisin Carlsberg containing 0.5-6.5 Anson Units of proteinase per gram of concentrate and method for preparing same. Solid form proteinase is extracted with 70-100 parts by volume propylene glycol, 30-0 parts by volume of water, then adjusted as necessary to 60-85% by wt. of the glycol. Stabilizing agents in the concentrate are 0.1-1 mol/kg. of one or more C.sub.1 -C.sub.3 carboxylate, formate being preferred, and a calcium ion content of 0.04-0.5% by wt., pH range is 5-8.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.