Detecting intracellular antigens in swelled and fixed cells with labeled antibody
US4499183A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Dec 21, 1982 |
| Grant date | Feb 12, 1985 |
| Priority date | — |
| Expiry date | Dec 21, 2002 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/808
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Methods and reagent kits for the detection of intracellular antigens such as TdT and antinuclear antibodies. The methods involve swelling the cells with a hypotonic solution for increasing the localization of large molecular weight substances such as antibodies at intracellular antigenic sites, fixing the cells and reacting them with antibodies specific for the intracellular antigen to be detected. The cells are then further reacted with labeled antibody specific for the first antibody, washed to remove unreacted antibodies and the presence of label detected whereby the presence or absence of the intracellular antigen may be determined.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.