Bacterial production of heterologous polypeptides under the control of a repressible promoter-operator
US4499188A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | May 5, 1982 |
| Grant date | Feb 12, 1985 |
| Priority date | — |
| Expiry date | May 5, 2002 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S930/142
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A process for bacterially producing heterologous polypeptides, particularly those such as human IFN-.beta. that inhibit bacterial growth, in which bacteria that have been transformed to express the heterologous polypeptide under the control of a trp promoter-operator are cultivated in a known volume of medium containing an excess of a preferred carbon source such as glucose and a predetermined amount of tryptophan that corresponds approximately to the amount of tryptophan contained in the bacteria in the volume of medium at a predetermined elevated cellular density, whereby expression of the heterologous polypeptide is substantially repressed until the bacteria grow to approximately the predetermined elevated cellular density and is thereafter automatically derepressed to permit expression of the heterologous polypeptide.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.