Procedure for immunologic determination of procollagen peptide (type III) and procollagen peptide col 1 (type III) together and a process for preparing anti-procollagen peptide col 1 (type III) serum

US4504587A · kind A · utility

Assignee

• Hoechst GmbH · DE

Inventors

• Rupert Timpl · Gauting, DE
• Dietrich Brocks · Wiesbaden, DE
• Horst Neubauer · Königstein, DE
• Helmut Strecker · Seeheim-Jugenheim, DE

Key dates

• Filing date: Mar 11, 1983
• Grant date: Mar 12, 1985
• Expiry date: Mar 11, 2003

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S436/824
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The antigens procollagen peptide (type (III) and procollagen peptide col 1 (type III) can be determined together immunologically by either PA0 (a) reacting a specified amount in each case of labeled procollagen peptide (type III) or procollagen peptide col 1 (type III) and a highly specific antiserum containing antibodies having affinity for both the antigens mentioned together with a sample having an unknown content of procollagen peptide (type III) and/or procollagen peptide col 1 (type III), separating off the antigen-antibody complex formed and measuring the amount of labeling in the complex and/or in the supernatant, or PA0 (b) bringing a specified amount of the highly specific antiserum to reaction with a sample having an unknown content of procollagen peptide (type III) and/or procollagen peptide col 1 (type III), fixing the unreacted amount of the antibody to procollagen peptide (type III) or procollagen peptide col 1 (type III) bound to a support, and bringing to reaction with a labeled second antibody, and then determining the amount of bound or excess second antibody by measuring the labeling. An anti-procollagen peptide col 1 (type III) serum is preferred as the highly …