Heterogeneous immunoassay method
US4506009A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 30, 1982 |
| Grant date | Mar 19, 1985 |
| Priority date | — |
| Expiry date | Mar 30, 2002 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/975
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A conjugate useful in determining the amount of antigen or antibody in a liquid sample, said conjugate having a marker, an immunoreactive component (i.e. antigen or antibody) bound to the marker and an insolubilizing binding component which is also bound to the marker. The insolubilizing binding component portion of the conjugate will react with an insolubilizing receptor to form a solid product of conjugate and receptor unless the conjugate reacts with the corresponding antigen or antibody to be analyzed in which event the conjugate will not react with the insolubilizing receptor. The conjugate will be added to a liquid sample containing an unknown amount of, for example, an antibody. A known amount of the corresponding antigen is also added which reacts with both the conjugate and antibody. After the reaction is complete, the liquid sample is contacted with the insolubilizing receptor. Since only the free conjugate reacts with the insolubilizing receptor the amount of antibody originally in the liquid sample can be determined by measuring the activity of the marker in the precipitate.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.