Chromogenic compounds, a process for their preparation and their use

US4508644A · kind A · utility

Assignee

• Behringwerke Aktiengesellschaft · DE

Inventors

• Helmut Heber · Marburg, DE
• Reinhard Eberle · Lahntal, DE
• Volker Teetz · Hofheim, DE

Key dates

• Filing date: Nov 25, 1983
• Grant date: Apr 2, 1985
• Expiry date: Nov 25, 2003

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S930/28
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Chromogenic compounds of the formula I ##STR1## and a process for their preparation are described, X denoting a hydrogen atom, a protective group customarily used in peptide chemistry or a group which irreversibly blocks the terminal amino group, PA0 A and P being identical or different and denoting an alpha-, beta- or gamma-amino acid which is composed of 2 to 15 carbon atoms with up to 3 nitrogen atoms, 2 sulfur atoms and 6 oxygen atoms, the side-chain of which can be substituted, and P also denoting a dipeptide formed from amino acids of these types, PA0 B denoting arginine, homoarginine, lysine, homolysine, ornithine or histidine and PA0 R denoting a substituent in the 2- or 3-position. The compounds can be used as substrates for the detection and the quantitative determination of hydrolytic enzymes of enzyme class 3.4.21.