Simultaneous assay for T and B lymphocyte populations and subpopulations
US4511662A · kind A · utility
100Cited by
13References
26Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jun 18, 1982 |
| Grant date | Apr 16, 1985 |
| Priority date | — |
| Expiry date | Jun 18, 2002 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/56972
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
An assay for simultaneously determining the ratio of B cells and T cells to the total cell population and subpopulations thereof present in a lymphocyte population utilizes excess amounts of B cell binding protein and T cell binding protein bound to solid phase particles. By exposing the particles to the lymphocyte population, rosettes are formed which may be visually distinguished and counted under a microscope, yielding the proportions of B cells and T cells, including any subpopulations for which a specific label was provided. Macrophages may be identified by their ability to ingest the particles.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.