Method and reagent for the enzymatic determination of enzyme substrates

US4517287A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Peter Scheibe · Munich, DE
• Erich Bernt · Munich, DE
• Sigmar Klose · Starnberg, DE

Key dates

• Filing date: Mar 25, 1980
• Grant date: May 14, 1985
• Expiry date: Mar 25, 2000

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/60
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention provides a reagent for the enzymatic determination of an enzyme substrate, containing a system for the determination of the substrate, which comprises at least one enzyme, at least one buffer substance and at least one indicator substance and optionally contains adjuvants, wherein the reagent contains a definite amount of the enzyme substrate in an insufficient amount with regard to the substrate determination capacity of the system for the determination of this substrate. The present invention also provides a process for the enzymatic determination of an enzyme substrate in the presence of disturbing substances which can react with the substrate itself or with an intermediate or end product of the indicator reaction, wherein, before the addition of the sample to be investigated, a definite amount of the substrate is first reacted with the reagent until reaction is complete, whereafter the sample to be determined is added.