Reduction in non-specific interference in hydrophobic ligand assays
US4518701A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jun 2, 1983 |
| Grant date | May 21, 1985 |
| Priority date | — |
| Expiry date | Jun 2, 2003 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/825
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Methods are provided for reducing non-specific interference in competitive protein binding assays employing as the labeled reagent a fluorescent conjugate of a hydrophobic ligand conjugated to a fluorescer, which in turn is bound to a water soluble polysaccharide carrier ("fluorescer conjugate reagent"). In order to reduce non-specific interference from physiologic samples, the fluorescent reagent is combined with a lipid substituted neutral support, under conditions which provides two binding fractions: a first weakly binding fraction which is relatively free of non-specific interference in a competitive protein binding assay employing physiological fluids; and a second fraction, which more strongly binds to the lipid substituted support.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.