Microbial production of indigo
US4520103A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Oct 27, 1982 |
| Grant date | May 28, 1985 |
| Priority date | — |
| Expiry date | Oct 27, 2002 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/877
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Microbial synthesis of indigo dyestuff in indole-free media is disclosed. Indigo production is preferably accomplished by genetic transformation of selected host cells having the capacity to produce and accumulate indole (either as a result of endogenous genomic capacity or genetic transformation) to incorporate the capacity for synthesis of an aromatic dioxygenase enzyme. Growth of transformed cells under suitable conditions facilitates aromatic dioxygenase enzyme catalyzed oxidative transformation of cellular indole, with consequent formation of indigo from the oxidized reaction products. In a highly preferred embodiment, E. coli cells having endogenous indole production capacity are transformed with a DNA expression vector comprising the structural gene for naphthalene dioxygenase, resulting in the microbial synthesis of isolatable quantities of indigo.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.