Fluorescent immunoassay employing a phycobiliprotein labeled ligand or receptor

US4520110A · kind A · utility

Assignee

• The Board of Trustees of the Leland Stanford Junior University · US

Inventors

• Lubert Stryer · Stanford, US
• Alexander N. Glazer · Orinda, US
• Vernon T. Oi · Beaumont, US

Key dates

• Filing date: Dec 30, 1982
• Grant date: May 28, 1985
• Expiry date: Dec 30, 2002

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S530/825
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Sensitive detection techniques and compositions for such techniques employing fluorescent proteins having bilin prosthetic groups as labels i.e. phycobiliprotein. The bilin containing proteins can be conjugated to ligands or receptors for use in systems involving ligand-receptor binding for the analysis, detection or separation of ligands and receptors. Particularly, one or more of the bilin containing proteins may be used as labels in conjuction with each other or other fluorescers for defining subsets of naturally occurring aggregations e.g. cells.