Patent · US Expired

Method for producing a preparation containing factor VIII (AHF)

US4522751A · kind A · utility

27Cited by

10References

10Claims

0Family size

Assignee

Immuno Aktiengesellschaft fur chemisch-medizinische Produkte · AT

Inventors

Yendra Linnau · Wien, AT
Otto Schwarz · Waldshut-Tiengen, DE

Key dates

Filing date	May 18, 1984
Grant date	Jun 11, 1985
Priority date	—
Expiry date	May 18, 2004

Classification

Technology area (CPC Y)Emerging Cross-Sectional Technologies
CPC primaryY10S530/83
WIPO fieldPharmaceuticals
WIPO sectorChemistry

Abstract

There is described a method for producing a preparation having a high content of Factor VIII (AHF), i.e. with a specific activity of at least 1.5 units of Factor VIII/mg protein, immunoglobulin G (IgG) of from 15 to 30 mg/1000 units of Factor VIII and fibrinogen of from 20 to 40 mg/100 units of Factor VIII. The method consists in that a Factor VIII containing plasma fraction is dissolved in a buffer, the solution is purified from undesired proteins by precipitation and is concentrated, the precipitation of undesired proteins being carried out in the presence of sulfated polysaccharide at a pH of from 6 to 7. After separation of the undesired proteins, a Factor VIII concentrate is precipitated, dissolved and processed into stable form. If desired, an antithrombin III-heparin complex or an antithrombin III-heparinoid complex is added to the solution.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.