Method of preparing highly purified, stroma-free, non-hepatitic human and animal hemoglobin solutions

US4526715A · kind A · utility

Assignee

• Biotest Pharma GmbH · DE

Inventors

• Norbert Kothe · Kronberg, DE
• Bertram Eichentopf · Bad Soden, DE

Key dates

• Filing date: May 29, 1984
• Grant date: Jul 2, 1985
• Expiry date: May 29, 2004

Classification

• Technology area (CPC A): Human Necessities
• CPC primary: A61K38/00
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

To provide a method that allows the preparation, not only from human blood but also from animal blood and in quantities large enough for clinical applications, of highly purified hemoglobin solutions that can be employed for the treatment of humans and that are free of plasma proteins and residual stroma lipids (a) a starting material that contains erythrocytes is pumped over a filter unit that has a pore size between 0.1 and 1.2 .mu.m in circulation against an isotonic washing solution, (b) the product of (a) is hemolyzed and pumped over an ultrafiltration unit that has a permeability of 80,000 to 100,000 D in circulation against water, and (c) the ultrafiltrate obtained in stage (b) is pumped and diafiltered over an ultrafiltration unit that has a permeability of 10,000 to 50,000 D in circulation against water, whereby concentration is conducted before and/or after diafiltration.