Multi-purpose blood diluent and lysing agent for differential determination of lymphoid-myeloid population of leukocytes
US4528274A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 25, 1983 |
| Grant date | Jul 9, 1985 |
| Priority date | — |
| Expiry date | Mar 25, 2003 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/108331
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method and reagent system is described for differential determination of more that one class of leukocyte populations, using an automatic blood cell analyzer. The reagent system includes a blood diluent and stromatolyzing reagent. The blood diluent is an osmotically balanced aqueous solution of ingredients for maintaining erythrocyte morphology, blood cell stabilizing, buffering and bacteriostatic action. The stromatolyzing reagent is a mixture of an aqueous solution of quaternary ammonium salts, a chromagen forming agent and an additive which is a non-cationic surfactant. The quaternary ammonium salts, with the additive, serve the purpose of positioning, relative to one another and a volume reference point, the lymphoid and myeloid populations. The additive also acts to prevent or to reduce the amount of protein deposit in the sensing orifices of the analyzer, which deposit tends to accumulate from the cell debris resulting from blood cell stromatolyzation.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.