Controlled oxidation of microbially produced cysteine-containing proteins
US4530787A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Oct 17, 1984 |
| Grant date | Jul 23, 1985 |
| Priority date | — |
| Expiry date | Oct 17, 2004 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S930/142
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Method of oxidizing reduced cysteine-containing microbially produced synthetic proteins, such as synthetic IFN-.beta. or synthetic IL-2, in a controlled manner so that the synthetic proteins have the same disulfide bridging as their native counterparts. The oxidation employs o-iodosobenzoate as oxidizing agent and is carried out in an aqueous medium at a pH at least about one-half pH unit less than the pK.sub.a of the cysteines to be oxidized, a synthetic protein concentration of less than about 5 mg/ml, and an oxidizing agent:protein mol ratio that is at least stoichiometric, provided that the oxidizing agent is in excess in the terminal portion of the reaction.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.