Patent · US Expired

Process for the injection of very small amounts of samples into cells

US4532205A · kind A · utility

3Cited by
2References
10Claims
0Family size

Assignee

Inventor

Key dates

Filing dateJan 21, 1983
Grant dateJul 30, 1985
Priority date
Expiry dateJan 21, 2003

Classification

  • Technology area (CPC Y)Emerging Cross-Sectional Technologies
  • CPC primaryY10T137/87265
  • WIPO fieldMeasurement
  • WIPO sectorInstruments

Abstract

A method and a device are proposed for the injection of very small amounts of samples into cells (74) with the aid of a microcapillary (10). During the injection, a constant injection pressure of about 0.12 bar, provided by a pressurized cylinder (46) is applied to the microcapillary (10), which is reduced after the completion of the injection to a holding pressure of 0.02 bar until the next cell (64) is to be injected. A high-pressure (p.sub.1) supplied from another pressurized cylinder (44) serves for blowing out the microcapillary tip (12). A three-way valve (42) permits simple and rapid switching between the various pressures (p.sub.1, p.sub.2 and p.sub.3). Reproducible amounts of sample in the range from 10.sup.-11 ml to 10.sup.-13 ml can be injected reproducibly, simply and rapidly.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.