Production of a cephalosporin by fermentation
US4533632A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Aug 6, 1982 |
| Grant date | Aug 6, 1985 |
| Priority date | — |
| Expiry date | Aug 6, 2002 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC02F3/30
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A process for greatly improving the yields of cephalosporin nucleus produced by fermentation is described. Cephalosporin C-producing microorganisms are fermented in the presence of an acetylesterase enzyme so that cephalosporin C formed is immediately converted into desacetyl cephalosporin C before any non-enzymic degradation occurs. Fermentation to produce desacetyl cephalosporin C enables yield increases of cephalosporin nucleus of about 40% to be realized, and greater increases are possible if the fermentation is extended. The preferred cephalosporin C-producing organism is Acremonium chrysogenum, and mutants thereof that are capable of producing esterases in situ. Alternatively, esterases obtained from a variety of other sources may be added or formed in situ, for example from a strain of Rhodosporidium.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.