Patent · US Expired

Tissue culture medium

US4533634A · kind A · utility

77Cited by
6References
9Claims
0Family size

Assignee

Inventors

Key dates

Filing dateJan 26, 1983
Grant dateAug 6, 1985
Priority date
Expiry dateJan 26, 2003

Classification

  • Technology area (CPC Y)Emerging Cross-Sectional Technologies
  • CPC primaryY10S530/83
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

A growth factor containing serum is disclosed which is derived from natural bovine serum is disclosed which is by precipitating a first solids fraction from natural bovine serum by adding ammonium sulfate to about 25% saturation to give a first solids fraction and a first supernatant; separating the first solids fraction from the first supernatant; precipitating a second solids fractions from the first supernatant by adding ammonium sulfate to about 40% saturation to give a second solids fraction and a second supernatant; separating the second solids fraction from the second supernatant; combining the first solids fraction and the second supernatant; and desalinating the first solids fraction and the second supernatant, either before or after combination thereof.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.