Static cell culture maintenance system
US4537860A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Dec 8, 1982 |
| Grant date | Aug 27, 1985 |
| Priority date | — |
| Expiry date | Dec 8, 2002 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12M29/04
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method and apparatus for maintaining animal cells in vitro in a substantially arrested state of proliferation with continuous secretion of cell product is disclosed. The cells are retained within a reactor vessel chamber in a semi-rigid matrix having interstices for passage of fluid nutrient medium. Fresh nutrient medium is supplied by perfusion into the matrix through relatively low porosity tubes which are suspended in the reactor chamber and which substantially traverse the matrix; expended medium and cell product is withdrawn through relatively high porosity tubes which also are suspended in the reactor chamber and which substantially traverse the matrix; and oxygenated gaseous medium is supplied by perfusion into the matrix through a selectively-permeable membrane disposed in the reactor chamber.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.