Process and reagent for the determination of creatinine

US4543325A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Winfried Albert · Eberfing, DE
• Joachim Ziegenhorn · Starnberg, DE
• Joachim Siedel · Bernried, DE
• Hans-Georg Batz · Tutzing, DE
• Helmut Lenz · Tutzing, DE
• Brigitte Pautz · Herrsching am Ammersee, DE

Key dates

• Filing date: Dec 15, 1982
• Grant date: Sep 24, 1985
• Expiry date: Dec 15, 2002

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S436/822
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The present invention provides a process for the immunological determination of creatinine, wherein creatinine is converted into 1-methylhydantoin, the 1-methylhydantoin formed is incubated in an aqueous medium with antibodies which are directed against a conjugate of a first hydantoin derivative of the general formula: ##STR1## in which R.sup.1, R.sup.2, R.sup.3 and R.sup.4, which can be the same or different, are hydrogen atoms, alkyl radicals containing up to 3 carbon atoms or phenyl radicals, with a first hapten carrier substance suitable for antibody formation, reacted with a conjugate of a second hydantoin derivative of general formula (I) with a second hapten carrier substance, one of the components antibody and conjugate being present in the solid phase or in dissolved form and the other component being present in dissolved form, and the inhibition of the binding reaction between the antibodies and the hydantoin conjugate with the second hapten carrier substance is measured. The present invention also provides a reagent for the immunological determination of creatinine, wherein it contains creatinine iminohydrolase, antibodies against a conjugate of a hydantoin of the gener…