Patent · US Expired

Liquid proteinase concentrate and method for preparation

US4543333A · kind A · utility

9Cited by
8References
6Claims
0Family size

Assignee

Inventors

Key dates

Filing dateJun 5, 1984
Grant dateSep 24, 1985
Priority date
Expiry dateJun 5, 2004

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC11D3/38663
  • WIPO fieldBasic materials chemistry
  • WIPO sectorChemistry

Abstract

A storage stable liquid enzyme concentrate of Subtilisin Carlsberg containing 0.5-6.5 Anson Units of proteinase per gram of concentrate and method for preparing same. Solid form proteinase is extracted with 70-100 parts by volume propylene glycol, 30-0 parts by volume of water, then adjusted as necessary to 60-85% by wt. of the glycol. Stabilizing agents in the concentrate are 0.1-1 mol/Kg of a member selected from the group consisting of Na, K, and Ca glutamates, and glycinates, and acetamide, and also a calcium ion content of 0.04-0.5% by wt., pH range is 5-8.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.