Device and method for the quantitative determination of heparin in mammalian blood plasma
US4543335A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Dec 20, 1982 |
| Grant date | Sep 24, 1985 |
| Priority date | — |
| Expiry date | Dec 20, 2002 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2400/40
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A device for the quantitative determination of heparin in mammalian blood plasma, a method for the preparation of such device and a method for determining the plasma's heparin concentration using the device. The device, which is prepared using a freeze-drying technique, comprises a first upper layer of a carrier matrix material containing a buffer, AT-III and thrombin and a second, lower layer of a carrier matrix containing a thrombin sensitive fluorogenic or chromogenic substrate being capable of interacting with thrombin in such a manner that a time-related chemical change detectable by fluorometric or spectrophotometric means takes place when thrombin and the substrate are contacted in a suitable liquid environment.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.