Process and reagent for the determination of .alpha.-amylase

US4544631A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Elli Rauscher · Munich, DE
• Ulrich Neumann · Peißenberg, DE
• August W. Wahlefeld · Hohenpeißenberg, DE
• Alexander Hagen · Tutzing, DE
• Wolfgang Gruber · Bischofswerda, DE
• Joachim Ziegenhorn · Starnberg, DE
• Eugen Schaich · Weilheim, DE
• Ulfert Deneke · Fürth, DE
• Gerhard Michal · Tutzing, DE
• Gunter Weimann

Key dates

• Filing date: Oct 16, 1981
• Grant date: Oct 1, 1985
• Expiry date: Oct 16, 2001

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S435/81
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

.alpha.-Amylase is determined by the enzymatic splitting of an .alpha.-amylase substrate and measurement of a fission product, wherein there is used as a substrate a maltoheptaose compound of the formula ##STR1## wherein R is a glucoside, phenylglucoside, mononitrophenylglucoside, dinitrophenylglucoside, sorbitol or gluconic acid group. Reagents comprising such a substrate and a system for the determination of a fission product formed from the amylase substrate by .alpha.-amylase, are also provided.