Process for producing vitamin B.sub.12 by the fermentation technique, and vitamin B.sub.12 -producing microorganism
US4544633A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Feb 23, 1983 |
| Grant date | Oct 1, 1985 |
| Priority date | — |
| Expiry date | Feb 23, 2003 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/822
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A process for producing vitamin B.sub.12 by the fermentation technique which comprises cultivating a vitamin B.sub.12 -producing microorganism belonging to the genus Propionibacterium in a culture medium containing a carbon source and a nitrogen source, and collecting vitamin B.sub.12 accumulated in the cells of the microorganism; characterized in that PA0 (1) the cultivation is carried out while adding an alkali at suitable times to the cultivation system so that during the cultivation, the cultivation system is maintained at a pH in the range of about 5 to about 7.5, and PA0 (2) the cultivation is carried out while adding a carbon source portionwise to the cultivation system nearly at the same time as the addition of the alkali, PA0 (3) provided that when a vitamin B.sub.12 -producing microorganism having resistance to propionic acid is used as the microorganism, the portionwise addition of the carbon source in (2) can be omitted. Also disclosed are propionic acid-resistant, vitamin B.sub.12 -producing microorganism of the genus Propionibacterium.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.