Enzymatic process for the determination of beta-lactam antibiotics
US4546076A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jan 31, 1983 |
| Grant date | Oct 8, 1985 |
| Priority date | — |
| Expiry date | Jan 31, 2003 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2415/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
An enzymatic process for the determination of beta-lactam antibiotics in a biological liquid, comprising the steps of: (1) incubating the liquid with soluble D-alanyl-D-alanine-carboxypeptidase produced by Actinomadura R 39, said enzyme being immobilized on a water-insoluble support, the beta-lactam antibiotic reacting with the immobilized enzyme to form an inactive and equimolecular enzyme-antibiotic complex; (2) separating the immobilized enzyme from the liquid and washing it; (3) incubating the immobilized enzyme with a substrate solution to hydrolyze it and form an amount of D-alanine corresponding to the residual enzymatic activity; (4) determining the amount of D-alanine formed; and (5) comparing the determination of step (4) with a standard to obtain the concentration of the antibiotic in the biological liquid; as well as a test set for carrying out this process and comprising the necessary reagents.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.