Multizone analytical element and method for analyte determination
US4547460A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Apr 16, 1984 |
| Grant date | Oct 15, 1985 |
| Priority date | — |
| Expiry date | Apr 16, 2004 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/25125
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A multizone analytical element for the determination of an analyte in an aqueous liquid comprises a registration zone and a porous spreading zone to receive and transport the aqueous liquid. The spreading zone contains a nonpolymeric quaternary ammonium compound represented by either of the structures: ##STR1## wherein represents a single or double bond; R.sub.1, R.sub.2, R.sub.3 and R.sub.4 are independently alkyl; aryl; cycloalkyl; or alkaryl; provided that R.sub.1, R.sub.2, R.sub.3 and R.sub.4 together comprise at least 12 carbon atoms; or R.sub.1 and R.sub.2 taken together with the nitrogen atom, form a quaternary 5- to 16-membered heterocyclic ring; or R.sub.1, R.sub.2 and R.sub.3 taken together with the nitrogen atom, form a quaternary 5- to 16-membered heterocyclic ring; and X.sup.- is a monovalent anion. This element is particularly useful for rate determinations of enzymes, such as alanine aminotransferase and aspartate aminotransferase, in liquids containing bilirubin or protein which may interfere with the accuracy of the assay.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.