Reagent composition, dry element and method for determination of total bilirubin
US4548905A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Apr 9, 1984 |
| Grant date | Oct 22, 1985 |
| Priority date | — |
| Expiry date | Apr 9, 2004 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/146666
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A reagent composition, dry analytical element and a method of using same to determine total bilirubin in aqueous liquids are disclosed. The reagent composition comprises a diazonium salt, or reagents capable of producing a diazonium salt when contacted with water, and 3,3-dimethylglutaric acid or a salt thereof present in an amount effective to buffer the composition at a pH of about 3.5 or less when the composition is contacted with a 2 .mu.L sample of pooled human serum. The composition and elements described herein provide a means to eliminate the effect of an undetermined interferent which is either preformed or formed in situ in diazo-based total bilirubin determinations of biological fluids (e.g. uremic serum).
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.