Rapid assaying method for guanase
US4550076A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Sep 27, 1982 |
| Grant date | Oct 29, 1985 |
| Priority date | — |
| Expiry date | Sep 27, 2002 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/978
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The guanase activity in body fluids such as blood serum can be rapidly and accurately assayed by (I) decomposing guanine with the guanase in the specimen to xanthine and ammonia at pH 7-9, preferably at pH 8, (II) decomposing the xanthine formed by former step I with xanthine oxidase to uric acid and hydrogen peroxide, (III) reacting the reactant solution of the former step II with 3-methyl-2-benzothiazolinonehydrazone, an aniline derivative such as N,N-di-lower-alkylaniline and peroxidase, and finally measuring the optical absorption of the reactant solution of the step III at 570-600 nm. The all steps can be completed within 15 minutes. Therefore, this assay is adoptable for automatic assay of guanase on usual clinically available automatic analyzers.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.