Use of pyrylium and thiapyrylium compounds as biological stains
US4555396A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Dec 22, 1982 |
| Grant date | Nov 26, 1985 |
| Priority date | — |
| Expiry date | Dec 22, 2002 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/5005
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Disclosed herein is an improvement in a method for distinguishing cells in a biological sample by staining with a dye, wherein the improvement comprises employing as the dye a compound of the formula ##STR1## wherein G is O or S; PA0 R.sup.1, R.sup.3, and R.sup.5 are independently selected from the group consisting of hydrogen, alkyl, aryl, aralkyl, amino, styryl, bis(diaryl)vinylene, and ##STR2## wherein R is hydrogen or alkyl; PA0 Z represents the elements necessary to complete a basic heterocyclic ring system of the type used in cyanine dyes; PA0 n is 0 or 1; PA0 R.sup.2 is hydrogen or, taken together with either R.sup.1 or R.sup.3, represents the elements needed to complete an aromatic or a carbocyclic ring system; PA0 R.sup.4 is hydrogen or, taken together with either R.sup.3 or R.sup.5, represents the elements needed to complete an aromatic or a carbocyclic ring system; and PA0 X.sup.- is an anion.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.