Analytical element and method for alkaline phosphatase assay
US4555484A · kind A · utility
15Cited by
8References
20Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jul 25, 1983 |
| Grant date | Nov 26, 1985 |
| Priority date | — |
| Expiry date | Jul 25, 2003 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/805
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed herein is a dry analytical element and a method of using same for quantitatively detecting alkaline phosphatase in an aqueous liquid. The element comprises, in fluid contact, first and second reagent zones which can be self-supporting or carried on a support. The first reagent zone contains a substrate for alkaline phosphatase, e.g. p-nitrophenyl phosphate, and the second reagent zone contains a buffer which is an alkali metal or ammonium salt and has a pKa in the range of 9-11.5, e.g. an alkali metal salt of carbonic acid.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.