Method for using an amino-terminus DNA sequence to synthesize a specific double-stranded DNA
US4555486A · kind A · utility
11Cited by
1References
12Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Feb 28, 1983 |
| Grant date | Nov 26, 1985 |
| Priority date | — |
| Expiry date | Feb 28, 2003 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K14/62
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method is described for constructing double-stranded DNA using messenger RNA templates. DNA strands complementary to the templates are formed using a non-specific 3' hydroxy terminus primer. After separation, the DNA single strands are disabled from self priming by blocking the free 3' hydroxy. Primer segments are then provided complementary to a portion of the amino terminus-coding end of the desired single strands. These primer segments are then extended with a suitable polymerase to complete the complementary strands.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.