Calibrated reaction measurement system
US4558013A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Apr 8, 1983 |
| Grant date | Dec 10, 1985 |
| Priority date | — |
| Expiry date | Apr 8, 2003 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/809
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Method and apparatus for use in accurately measuring the binding reactions occurring between a predetermined class of components in a liquid specimen and their corresponding binding conjugates coated on separate test regions of a carrier. The carrier further includes a reference region that is uncoated, and the carrier is adapted for simultaneous contact of all of its regions with the liquid specimen. Any resulting binding reaction on each test region indicates both specific binding to the coated component as well as non-specific binding, and any resulting binding reaction on the reference region indicates merely non-specific binding. The measurements for the test regions are all reduced by an amount determined in accordance with the measurement for the reference region, to reduce the effects of non-specific binding on those measurements and thereby provide accurate measurements of the specific binding on each coated component.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.