Assay methods and systems utilizing mast cell clones
US4559310A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | May 20, 1983 |
| Grant date | Dec 17, 1985 |
| Priority date | — |
| Expiry date | May 20, 2003 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/808
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention is directed to an in vitro assay, useful in determining the effectiveness of anti-allergy compounds and/or useful in measuring the degree of sensitivity of a patient to particular allergens. The present invention permits potential anti-allergy agents to be assayed in a number of ways. For example, the binding and dissociation rates of IgE to the mast cells in the presence and the absence of the substance being tested may be measured thereby giving a direct indication of that substance's ability to interfere with the IgE binding reaction. Another measure of a substance's potential as an anti-allergy agent is based upon the release of mediators or other compounds from the mast cells after sensitization by the allergen and exposure of the sensitized cells to the allergen. The present invention generally involves the following steps: PA1 (a) sensitizing cloned mast cells to an allergen; PA1 (b) exposing sensitized mast cells to the allergen in the presence of a test anti-allergy agent; and PA1 (c) measuring the reaction products of step (b) for an indication of test compound effect.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.