Radiolabelled substrates for assaying mammalian enzymes

US4563305A · kind A · utility

Assignee

• The University of Miami · US

Inventors

• James W. Ryan · Augusta, US
• Alfred Chung · Miami, US

Key dates

• Filing date: Jan 7, 1981
• Grant date: Jan 7, 1986
• Expiry date: Jan 7, 2001

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S930/28
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Compounds which are substrates for mammalian enzymes, especially proteolytic and peptidase enzymes, are disclosed. These compounds, in general, have the general formula X-T, W-Y or X-(Pep)-Y, wherein X is a radiolabelled benzoyl or hydroxyphenylpropanoyl group, T is the --NH residue of a known chromogenic or fluorogenic substrate comprising at least one amino acid group which may be substituted with other organic groups, Y is a radiolabelled benzylamine group, W is the ##STR1## residue of a known chromogenic or fluorogenic substrate comprising at least one amino acid group which may be substituted with other organic groups, and (Pep) is a difunctional or polyfunctional residue from a known chromogenic or fluorogenic substrate comprising at least two amino acid groups in peptide linkage, which residue may be substituted with other organic groups. These substrates exhibit many advantages over the chromogenic and fluorogenic analogs, including greater sensitivity, surprising and unpredictable affinity and specificity, and the like.