Method for fluorescence spectroscopic determination of a biologically active substance
US4565790A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jul 14, 1983 |
| Grant date | Jan 21, 1986 |
| Priority date | — |
| Expiry date | Jul 14, 2003 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/825
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Method for fluorescence spectroscopic determination of a biological substance provided with a marker consisting of a lanthanide chelate complex formed by a lanthanide coupled to the substance via a chelate forming compound, such as an EDTA-analogue, the lanthanide ion before the detection is dissociated from the active substance for instance by adding a buffer with a pH value below 3.5 and a splitting detergent, whereafter the excitation in the determination takes place in the solution in the presence of a .beta.-diketone by using a short radiation pulse and the fluorescence from the marker is detected when the fluorescence from the noise sources has substantially ceased.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.