Process for recovering microbially produced interleukin-2 and purified recombinant interleukin-2 compositions
US4569790A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 28, 1984 |
| Grant date | Feb 11, 1986 |
| Priority date | — |
| Expiry date | Mar 28, 2004 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61K38/00
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A process for recovering microbially produced IL-2 in a highly pure form from the cellular material of the microorganisms that produced it comprising: disrupting the cell membranes of the microorganisms; extracting the disruptate with a chaotropic agent, such as urea, that selectively extracts microbial proteins from the cellular material; solubilizing the IL-2 in the solid phase of the extraction mixture with an aqueous solution of a solubilizing agent, such as SDS, containing a reducing agent; and separating the IL-2 from the resulting solution by an optional extraction with 2-butanol or 2-methyl-2-butanol followed by gel filtration chromatography, oxidizing the IL-2 and purifying the oxidized IL-2 by RP-HPLC.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.