Method for the direct determination of the lipid content of blood beta-lipoproteins

US4569917A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Josef Maier · Eichstätt, DE
• Manfred Gloger · Weilheim, DE
• Brigitte Draeger · Tutzing, DE

Key dates

• Filing date: Mar 5, 1981
• Grant date: Feb 11, 1986
• Expiry date: Mar 5, 2001

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10T436/255
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Method of direct determination of beta-lipoproteins in a blood sample containing pre-beta-lipoprotein, (VLDL) alpha-lipoprotein (HDL), and beta-lipoprotein (LDL), comprising mixing the blood plasma or serum of the blood sample with a solid, water-insoluble anion exchanger operable to retain the pre-beta-lipoprotein (VLDL) and the alpha-lipoprotein (HDL) to remove them from solution, separating the blood plasma or serum from the anion exchanger and thereafter determining the amount of beta-lipoprotein (LDL) remaining in solution.