Patent · US Expired

Electrochemical sensor having an immobilized enzyme membrane

US4579642A · kind A · utility

30Cited by
2References
12Claims
0Family size

Assignee

Inventors

Key dates

Filing dateNov 7, 1984
Grant dateApr 1, 1986
Priority date
Expiry dateNov 7, 2004

Classification

  • Technology area (CPC Y)Emerging Cross-Sectional Technologies
  • CPC primaryY10S435/817
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

A recess of a predetermined thickness is formed in the bottom of a cylindrical vessel and a hole for the liquid-junction is formed at the center of the recess, to which is joined a resilient NH.sub.4.sup.+ selective membrane. Since the NH.sub.4.sup.+ selective membrane has a thickness larger by about 0.1 mm than the depth of the recess, the NH.sub.4.sup.+ selective membrane is protruded out of the bottom of the vessel by so much. A polyester woven fabric on which urease is immobilized is attached to the vessel so as to cover the NH.sub.4.sup.+ selective membrane. Since the immobilized enzyme membrane is applied with an appropriate tension by the slightly protruded ion selective membrane, bondability between the two membranes can be improved.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.