Method for fluorometric determination of the concentrations of substances in a sample and arrangement for implementing this method

US4580059A · kind A · utility

Assignee

• AVL AG · CH

Inventors

• Otto S. Wolfbeis · Regensburg, DE
• Edmund Urbano · Graz, AT

Key dates

• Filing date: Jun 6, 1984
• Grant date: Apr 1, 1986
• Expiry date: Jun 6, 2004

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10T436/209163
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

For the simultaneous measurement of the concentrations of several substances a number of fluorescence measurements corresponding to the number of substances to be tested are performed using at least one fluorescent indicator which is non-specific relative to at least one of the substances to be tested, each fluorescent indicator having different quenching constants with regard to the individual substances quenching its intensities. From the known, unquenched fluorescence intensities of the fluorescent indicators employed, the quenched fluroescence intensities obtained by measuring, and from the quenching constants that are known, or rather, have been determined beforehand by graphical methods or calculation, the concentrations and/or concentration ratios of the individual substances are obtained.