Novel alkaline protease
US4581332A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Sep 28, 1983 |
| Grant date | Apr 8, 1986 |
| Priority date | — |
| Expiry date | Sep 28, 2003 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/824
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A novel protease is described. The enzyme has the following characteristics: molecular weight: 30,000 (a gel filtration method using Sephadex G-75); isoelectric point, 5.3; pH reactivity: the optimum pH for its esterase activity is at pH 8.5 and the optimum pH for its amidase activity is at pH 9.0 respectively; substrate reactivity: it selectively and specifically hydrolyzes the ester bond and the amide bond derived from the carboxyl group of L-lysine; inhibitor: it is inhibited by diisopropyl phosphofluoride, tosyl-L-lysine chloro-methyl ketone and phenylmethylsulfonyl chloride. The protease is preferably produced by a microorganism belonging to the genus Achromobacter.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.