Simultaneous detection of leukocyte phagocytic and killing ability
US4581334A · kind A · utility
12Cited by
4References
13Claims
0Family size
Assignee
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Key dates
| Filing date | Apr 25, 1983 |
| Grant date | Apr 8, 1986 |
| Priority date | — |
| Expiry date | Apr 25, 2003 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/02
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Methods for the determination of phagocytic and killing ability of selected leukocyte subclasses based upon the use of three part differentials obtained by light scatter characteristics and gated fluorescence. Particles having multiple fluorescent labels associated therewith, one of which is sensitive to reactive oxygen, incubated with the leukocytes. Detection of the fluorescent labels permits identification of cells having phagocytic ability, as determined by presence of the non-sensitive label, and also the identification of those with killing ability as determined by the decrease in fluorescence from the sensitive label.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.