Immuno-agglutination particle suspensions
US4582810A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Sep 30, 1983 |
| Grant date | Apr 15, 1986 |
| Priority date | — |
| Expiry date | Sep 30, 2003 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/25875
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A suspension of diagnostic particles comprising antibody molecules attached to a carboxylate derivatized polymer core is provided for agglutination tests. The antibody is linked to the core through an avidin-biotin bridge. Avidin is joined by an amide bond to carboxyl groups on the core, and biotin is linked by an amide bond to amino groups on the antibody molecule. The core-bound antibody is exposed to a mixture of free biotin and biotinylated antibody to attach a controlled amount of antibody that is consistent with suspension stability prior to its use in a test and rapid cross-linking of suspended particles in the presence of antigen.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.