Method of activating hydroxyl groups of a polymeric carrier using 2-fluoro-1-methylpyridinium toluene-4-sulfonate
US4582875A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Dec 7, 1984 |
| Grant date | Apr 15, 1986 |
| Priority date | — |
| Expiry date | Dec 7, 2004 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/816
- WIPO fieldMacromolecular chemistry, polymers
- WIPO sectorChemistry
Abstract
Organic ligands containing at least one primary or secondary amino group or sulfhydryl group are coupled to a polymeric carrier containing at least one hydroxyl group, by activating the polymeric carrier by treatment with 2-fluoro-1-methylpyridinium toluene-4-sulfonate and reacting the activated polymeric carrier with the organic ligand which is thereby bonded directly to a carbon atom of the polymeric carrier. The polymeric carrier can, for example, be a possibly cross-linked polysaccharide and the organic ligand is preferably a biologically active material such as a drug or protein. The coupling product is an affinity matrix which can be used for affinity purification, covalent chromatography and reversible or irreversible covalent immobilization of biologically active molecules. The coupling method can be performed under mild conditions not damaging sensitive molecules.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.