Process for the purification of dextran-sucrase
US4591563A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | May 3, 1984 |
| Grant date | May 27, 1986 |
| Priority date | — |
| Expiry date | May 3, 2004 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/822
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention is directed to a process for the purification of the dextran-sucrase produced by Leuconostoc mesenteroides bacteria. The process according to the invention consists of adding to the culture medium, that contains the extra-cellular enzyme and dextran, a quantity of a PEG type polyether so that, in the medium appear two non miscible phases, that are thus maintained under stirring in order to obtain a good contact. Thereafter the lower dextran phase is separated from the upper polyether phase in order to provide a dextran-sucrase enriched enzymatic preparation. The purified enzyme can be used in the synthesis processes of the dextrans possessing specific molecular weights for certain applications.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.